DETERMINATION OF PESTICIDES IN VEGETABLES AND FRUIT FROM SUPERMARKETS OF LUCKNOW CITY USING GAS CHROMATOGRAPHY-MASS SPECTROSCOPY.
- Pesticide Toxicology Laboratory, Regulatory Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh 226001, India.
- Department of Chemistry, Babu Banarasi Das University, Faizabad Road, Lucknow Uttar Pradesh 226028, India.
- Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh 226001, India.
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Pesticide use becomes an integral part of modern agricultural practices and may cause the threat of its presence in different food commodities. In this context 31 pesticides were analyzed in different vegetables and fruit samples collected from different malls in Lucknow city, India. Extraction and cleanup of pesticide residues was done by QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method using ethyl acetate as a extraction solvent and different sorbents like primary and secondary amine, charcoal for dispersive solid phase cleanup of matrix component. Analysis of pesticide residues was done by using gas chromatography equipped with electron capture detector (GC-ECD) and detected pesticide was further confirmed by gas chromatography and mass spectrometry (GC-MS). Validated method was found accurate (% recovery 79-104%) and precise with relative standard deviation (%RSD) of less than 15. Limit of detection ranged from 1.49-5.25 mg kg-1 and limit of quantification ranged were 4.46-15.74 mg kg-1. In overall 270 analyzed samples of vegetable and fruits only 35 samples were detected with pesticide residues in which only 9 pesticides are found above MRL value according to CODEX/FSSAI MRL values. Pesticide residues were detected in cauliflower, cabbage, brinjal, green pea, broccoli, apple, grapes and banana.
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[Abhishek Kumar Singh, Anshuman Srivastava, Chandra Prabha Pandey and V. P sharma. (2017); DETERMINATION OF PESTICIDES IN VEGETABLES AND FRUIT FROM SUPERMARKETS OF LUCKNOW CITY USING GAS CHROMATOGRAPHY-MASS SPECTROSCOPY. Int. J. of Adv. Res. 5 (Oct). 1600-1607] (ISSN 2320-5407). www.journalijar.com
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